PhD Position in Single Cell Immunobiology bei Universitätsklinikum Freiburg

PhD Position in Single Cell Immunobiology (m/f/d) bei Universitätsklinikum Freiburg

Universitätsklinikum Freiburg

The Department of Internal Medicine II at the University Hospital in Freiburg is investigating the role of gamma delta T cells in intestinal inflammation and is looking for one for this purpose

PhD Position in Single Cell Immunobiology (m/f/d)

We are a small, dedicated group of scientists working to understand gamma delta T cell biology using cutting-edge single-cell technologies. We are seeking a highly motivated and skilled individual to join our team as a PhD student as part of the collaborative research center (CRC1160) to investigate the role of gamma delta T cells in intestinal inflammation in mice and humans.

https://www.orcid.org/0000-0002-8657-4704

https://www.uniklinik-freiburg.de/medizin2/forschung/ag-sagar.html

https://www.sfb1160.uni-freiburg.de

Project description

PhD position in Single-Cell Analysis of GAMMA DELTA T cell biology in INTESTINAL inflammation

The project involves substantial collaboration with other scientists of CRC 1160, who will perform experiments on DSS-induced colitis to investigate the immune landscape during inflammation in the intestine. The main aim of the project is to analyze single-cell multimodal datasets (including gene expression, chromatin accessibility, T-cell receptor sequencing, and cell-surface protein profiling) to understand the role of immune cells and their interactions with epithelial cells in intestinal homeostasis and inflammation, with a special focus on gamma delta T cells.

What we offer:

a four-year contract based on the German public service pay scale
a flexible environment where you will have the possibility to develop your own skillsets
mentorship on how to be productive and successful, resulting in several publications without micromanaging
joining date is flexible

Responsibilities:

perform single-cell RNA sequencing and other single-cell multimodal profiling using microfluidics and split and pool technologies
intensively perform computational data analysis of the generated datasets in collaboration with other scientists
present the biological interpretation of datasets in a comprehensive way during lab meetings, retreats, and meetings with collaboration partners

Requirements:

Master's degree in biology, molecular medicine, or immunology
experience in basic molecular and cellular biology techniques such as cell culture, FACS, qPCR, etc.
basic knowledge of R and/or Python
strong interest and motivation in analyzing your own datasets as the position is mainly computational
excellent communication skills in English
ability to work independently and to derive creative hypotheses from the data for further validation experiments

Required documents:

motivation letter and CV
Bachelor's and master's degree certificates
transcript records
minimum two letters of recommendation

Selection procedure:

presentation of Master's thesis research
presentation of a paper related to single-cell biology
the applicant will be given a sample dataset to analyze and will be asked to present the data

Please apply by sending all documents.

General information: Salary is assigned according to a pay scale. Unless prevented by operational or legal reasons, full-time positions are generally open to those wishing to job share. Where two candidates are equally suitable for a post, severely disabled candidates are given priority. Employment decisions are made by the Personnel Department.